How To Calculate Concentration Using Standard Addition Method

Complement the standard with many solutions.

The figure below shows some of the standard solutions used to add standards. The solvent is water and each solvent contains 25 ml of sample.Figure5.07_Flasks_StandardAddition.jpgRead: how to calculate concentration using standard addition

  • The first solution on the left has no standard. Why does it have color?

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  • From left to right, each solution contains 0.00, 1.00, 2.00, 3.00, 4.00 and 5.00 ml of 100.6 mg/l analyte solution. Does that explain why the solution on the far right is darker? Why?

    • Read more: How to draw dog hair with acrylic Read more: how to cook dal in the microwaveRead more: How to draw dog hair with acrylic in the microwave Read more: How to draw dog hair with acrylic Read more: how to cook dal in the microwave Read more: How to paint dog hair with acrylic Read more: how to cook dal in the microwave The mark of each solution is recorded and graphed against the concentration of each solution. When looking at the graph, why doesn’t the line intersect the y axis at 0 mg/l?Figure5.07b_CalibrationCurve.png

  • To calculate the concentration of the sample, the intercept x is calculated by substituting y = 0 into the straight-line equation (y = mx + b) and solving for x.
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    Sometimes plotting can be done using the mass of the added standard, rather than its concentration. In those cases, remember to convert the volume of the analyte in the sample to concentration. Read more: How to draw dog hair with acrylic Read more: how to cook dal in the microwave Read more: How to paint dog hair with acrylic dal in the microwave Read more: How to draw dog hair with acrylic Read more: how to cook dal in the oven microwave Read more: How to draw dog hair with acrylic Cooking dal in the microwave Read more: How to paint dog hair with acrylic how to cook dal in the microwave Read more: How to draw dog hair with acrylic Read more: how to cook dal in the oven microwave

  • Another spectrophotometric method for the determination of Pb2+ in blood requires a standard addition calibration curve. The standard was prepared by adding 1.00 ml of blood to each person, and the 1560 ppb external standard of Pb2+ was added. All samples were diluted to 5.00 ml before signal measurement. A standard curve of the signal against the mass of the added standard produces an equation of:[Signal = 0.266+312: mL^{-1}times V_{std} nonumber]
  • What is the concentration of Pb2+ in the blood sample? Read more: How to paint dog hair with acrylic Read more: how to cook dal in the microwave Acrylic dog fur Read more: how to cook dal in the microwave Read more: How to paint dog hair with acrylic Paint dog hair with acrylic Read more: how to cook dal in the microwave Read more: How to draw dog hair with acrylic how to paint dog hair with acrylic Read more: how to cook dal in the microwaveRead more: How to draw dog hair with acrylic
  • Can we use the same calibration curve for other blood samples? Read more: How to draw dog hair with acrylic Read more: how to cook dal in the microwave Read more: How to draw dog hair with acrylic
  • Can we use the same calibration curve to determine lead in seawater? Read more: How to draw dog hair with acrylic How to draw dog hair with acrylic Read more: how to cook dal in the microwave
  • What advantage does this calibration method have over direct calibration? Read more: How to draw dog hair with acrylic Read more: how to cook dal in the microwave Read more: How to draw dog hair with acrylic : How to draw dog hair with acrylic
  • What are the disadvantages of this calibration method when comparing calibrations? Read more: How to paint dog hair with acrylic Read more: how to cook dal in the microwave paint dog hair with acrylic Read more: how to cook dal in the microwave
  • Would you recommend this method to analyze any type of blood sample: adult, pediatric, fly blood? Consider sample size.
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